Single cell analysis of human tissues and solid tumors with mass cytometry
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- 作者：Nalin Leelatian ; Deon B. Doxie ; Allison R. Greenplate ; Bret C. Mobley ; Jonathan M. Lehman ; Justine Sinnaeve ; Rondi M. Kauffmann ; Jay A. Werkhaven ; Akshitkumar M. Mistry ; Kyle D. Weaver ; Reid C. Thompson ; Pierre P. Massion ; Mary A. Hooks ; Mark C. Kelley ; Lola B. Chambless ; Rebecca A. Ihrie and Jonathan M. Irish
- 刊名：Cytometry Part B: Clinical Cytometry
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：92
- 期：1
- 页码：68-78
- 全文大小：1012K
- ISSN：1552-4957
文摘
Mass cytometry measures 36 or more markers per cell and is an appealing platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While tissue disaggregation and fluorescence cytometry protocols were pioneered decades ago, it is not known whether established protocols will be effective for mass cytometry and maintain cancer and stromal cell diversity.