摘要
The groundwater and soil of Zhangshi irrigated place were seriously contaminated by organic pollutant. The PAHs (phenanthrene) was excessive. The harmony of the experimental conditions and the environment of research region was controlled. 20 strains with ability to degrade phenanthrene, added as the only carbon and energy source were isolated from the indigenous microorganisms of study area by selective enrichment and culture in laboratory. With the degradation test of 9 days , one strain was enriched in the condition that the temperature is 10 ℃ and the pH is 7.5, which the removal rates of phenanthrene (50 mg/L) were higher than 85 %. In the condition that the temperature is between 5 ℃ to 30 ℃ and the pH is between 6.0 to 9.0, the strain degraded phenanthrene by 96% within 15 days. The results provide technical basis for the application of degrading phenanthrene with in-situ biodegradation method which is in the groundwater and soil of Zhangshi cannal and other similar areas.