This study presents detailed microscopic observations for the Tongguanshan pluton distributed along the Yangtze River region in south Anhui Province. Using LA-ICP-MS method, trace elements and REE analyses for main rock forming minerals indicate that amphiboles may be mantle or crust-mantle mixing sources and plagioclase may form from the mixing of magma of different magmatic differentiation. The study positively reflects that during the formation of the Tongguanshan pluton, magma source and crystallization kinetic process, i.e. mixing metasomatism of crust and mantle, coincide with previous data using elements and isotopic means.space prefix = "o" ns = "urn:schemas-microsoft-com:office:office" />