Tests on zeta potential and flotation of pure minerals in collophanite were conducted to study the influence of Ca<sup>2+sup> and Mg<sup>2+sup> on collophanite surface electrical properties and flotability. The electro negative decreases because Ca<sup>2+sup> and Mg<sup>2+sup>are adsorbed preferentially on collophanite. However, collophanite is obviously depressed by Ca<sup>2+sup> and Mg<sup>2+sup> under alkaline condition, because that Ca<sup>2+sup> and Mg<sup>2+sup> might consume collector to precipitate. Otherwise, collophanite become hydrophilic because that calcium hydroxide and magnesium hydroxide absorption occurs on the surface.