The Huajian gold deposit is a typical quartz vein type gold deposit in eastern Hebei Province, and gold occurs mainly in polymetallic sulfide-quartz veins. Hydrogen and oxygen isotope studies suggest that at the ore-forming stage the mineralizing fluids were mainly magmatic water, but at the end of the ore-forming process the mineralizing fluids became mixed fluids mainly due to the addition of meteoric water. Characteristics of sulfur and lead isotopes show that Au mainly came from the anatexis of Archean metamorphic rocks. The direct and indirect sources of ore materials were Niuxinshan garnites and Archean Zunhua Group respectively. The mineralization was related to deep magmatic activation.