By measuring the stable carbon isotopes of satisfied hydrocarbon, aromatics and single hydrocarbons in crude oil collected from the Wunan oilfield in southwest margin of Qaidam basin, the authors discuss the geochemical features of the crude oil. The results indicate that the n-alkanes present a bimodal distribution pattern, associated with low ratios of Pr/Ph(<0.5) and C35-Hopane/C34-Hopane(<1.0), high abundance of tricyclic-terpanes and gammacerane, and occurrence of hopane (C35). These results confirm that the crude oils in the Wunan oilfield were formed in a mild-salty to salty reduction depositional environment, and the parent input matter mainly derived from the lower aquatic organisms.