The Huaniushan skarn-type gold deposit is located in the southern Beishan area. The ore bodies occur in the contact zone between the Huaniushan granite and the marble. Re-Os dating of a molybdenite sample yields a model age of 221.0-3.4 Ma, which is consistent with the zircon U-Pb age of the granite (225.6--2.2 Ma) within errors. The gold mineralization is therefore considered to be genetically related to the Huaniushan granite. Four main types of fluid inclusions are recognized: (1) two-phase liquid-rich inclusions, (2) two-phase gas-rich inclusions, (3) three-phase CH4-CO2-H20 inclusions, and (4) monophase vapor inclusions. Fluids associated with Au-bearing quartz veins (the early stage) have medium-high temperatures (285~C to 436~C) and moderate salinities (6.59% to 16.14% NaC1 equivalent); whereas fluids associated with calcite veins (the late stage) have low temperatures of 121 ~C to 182 ~C and low salinities of about 4.34% to 8.01% NaC1 equivalent. The temperature and salinity of the ore-forming fluids decrease progressively. Fluid boiling happened during the main Au mineralization period at the temperature of about 300℃ to 415℃. Oxygen and hydrogen isotopic compositions of the ore fluids range from -0.01‰ to 0.29‰ and -116‰ to -109‰, respectively, which is compatible with mixing between the magmatic and meteoric components. Measured 348 values for sulfide minerals are 4.21‰-to 6.53‰, indicating a magmatic sulfur origin. Considering the regional geology, we further propose that the Huaniushan skarn-type gold deposit was probably formed in the post-orogenic extensional setting.