long-chain alkenone (LCA) unsaturation proxies U Kor U K' The 37 have been widely applied for reconstructing past sea surface temperatures, but there are fewer studies on their applications in lacustrine system. Here we studied the distribution of LCA in a 5-m sediment core from the Kusai Lake, northern Tibetan Plateau, and the LCA-producing algae were determined by molecular biology methods. Results showed that LCA existed in most subsamples of the core with the ratio of C37/C38 〈1. The dominant haptophyte in the sediment core was closely related to Isochrysis according to DNA sequencing result, which was the LCA producer in the Kusai Lake. Base on the published temperature calibration of U Kfor lacustrine environment, the esti- mated paleo-lake water temperatures were close to the modern lake summer water temperature, and in accordance with the range of reconstructed paleotemperatures from Glycerol Dialkyl Glycerol Tetraethers (GDGT). However, in general the LCA- derived temperatures were slightly higher than the GDGT-derived temperatures, which is probably due to the fact that the or- ganisms producing the two groups of lipids lived at different depths of the lake water column.